Review



tfr 1 in 1  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    MedChemExpress tfr 1 in 1
    Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, <t>TfR-1-IN-1,</t> and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.
    Tfr 1 In 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tfr 1 in 1/product/MedChemExpress
    Average 94 stars, based on 2 article reviews
    tfr 1 in 1 - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "Targeting Piezo1 can improve endometrial fibrosis by inhibiting ferroptosis in endothelial cells"

    Article Title: Targeting Piezo1 can improve endometrial fibrosis by inhibiting ferroptosis in endothelial cells

    Journal: iScience

    doi: 10.1016/j.isci.2025.114540

    Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.
    Figure Legend Snippet: Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.

    Techniques Used: Activation Assay, Immunofluorescence, Iron Assay, Staining, Comparison



    Similar Products

    tfr 1 in 1  (MedChemExpress)
    94
    MedChemExpress tfr 1 in 1
    Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, <t>TfR-1-IN-1,</t> and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.
    Tfr 1 In 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tfr 1 in 1/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    tfr 1 in 1 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    human tfr 1 ecto domain  (Sino Biological)
    94
    Sino Biological human tfr 1 ecto domain
    Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, <t>TfR-1-IN-1,</t> and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.
    Human Tfr 1 Ecto Domain, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tfr 1 ecto domain/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    human tfr 1 ecto domain - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    tfrc inhibitor  (MedChemExpress)
    94
    MedChemExpress tfrc inhibitor
    Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, <t>TfR-1-IN-1,</t> and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.
    Tfrc Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tfrc inhibitor/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    tfrc inhibitor - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    α tfr antibody  (Proteintech)
    96
    Proteintech α tfr antibody
    Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, <t>TfR-1-IN-1,</t> and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.
    α Tfr Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α tfr antibody/product/Proteintech
    Average 96 stars, based on 1 article reviews
    α tfr antibody - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    tfr1 transferrin receptor protein 1  (MedChemExpress)
    94
    MedChemExpress tfr1 transferrin receptor protein 1
    Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, <t>TfR-1-IN-1,</t> and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.
    Tfr1 Transferrin Receptor Protein 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tfr1 transferrin receptor protein 1/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    tfr1 transferrin receptor protein 1 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    1 ap tfr proteintech  (Proteintech)
    96
    Proteintech 1 ap tfr proteintech
    Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, <t>TfR-1-IN-1,</t> and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.
    1 Ap Tfr Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 ap tfr proteintech/product/Proteintech
    Average 96 stars, based on 1 article reviews
    1 ap tfr proteintech - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    antibody against tfr-1 clone h68.4  (Thermo Fisher)
    90
    Thermo Fisher antibody against tfr-1 clone h68.4
    Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, <t>TfR-1-IN-1,</t> and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.
    Antibody Against Tfr 1 Clone H68.4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against tfr-1 clone h68.4/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    antibody against tfr-1 clone h68.4 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    antihuman tfr- 1 mouse monoclonal antibody #13- 6890  (Thermo Fisher)
    90
    Thermo Fisher antihuman tfr- 1 mouse monoclonal antibody #13- 6890
    Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, <t>TfR-1-IN-1,</t> and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.
    Antihuman Tfr 1 Mouse Monoclonal Antibody #13 6890, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antihuman tfr- 1 mouse monoclonal antibody #13- 6890/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    antihuman tfr- 1 mouse monoclonal antibody #13- 6890 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    antibody against tfr-1 #13-6890  (Thermo Fisher)
    90
    Thermo Fisher antibody against tfr-1 #13-6890
    Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, <t>TfR-1-IN-1,</t> and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.
    Antibody Against Tfr 1 #13 6890, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against tfr-1 #13-6890/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    antibody against tfr-1 #13-6890 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.

    Journal: iScience

    Article Title: Targeting Piezo1 can improve endometrial fibrosis by inhibiting ferroptosis in endothelial cells

    doi: 10.1016/j.isci.2025.114540

    Figure Lengend Snippet: Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.

    Article Snippet: TfR-1-IN-1 , MCE , Cat#HY-159569.

    Techniques: Activation Assay, Immunofluorescence, Iron Assay, Staining, Comparison